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recombinant human il 2 protein  (MedChemExpress)


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    Structured Review

    MedChemExpress recombinant human il 2 protein
    Recombinant Human Il 2 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il+2/IL-2%2C+Human/pmc13389675-136-27-31
    Average 93 stars, based on 6 article reviews
    recombinant human il 2 protein - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Vorinostat Potentiates Chemoimmunotherapy in Immune-Enriched Pancreatic Cancer.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples of 160 PDAC patients using a human lymphocyte separation tube (Dakewe Biotech, Cat# 7922112). .. Isolated PBMCs were activated with anti-human CD3/CD28 (Stemcell, Cat# 10971) and 30 U/mL recombinant human IL-2 (MCE, Cat# HY-P7037) for 24 h prior to co-culture. .. Levels of TNF- α and IFN- γ in supernatants from cocultures of PBMCs with Panc-1 cells were measured by ELISA (Cloud-Clone, Cat# USEA133Hu, Cat# USEA049Hu) to determine the cell ratio that best recapitulates an “immune-hot” cytokine milieu.

    Recombinant:

    Article Title: Vorinostat Potentiates Chemoimmunotherapy in Immune-Enriched Pancreatic Cancer.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples of 160 PDAC patients using a human lymphocyte separation tube (Dakewe Biotech, Cat# 7922112). .. Isolated PBMCs were activated with anti-human CD3/CD28 (Stemcell, Cat# 10971) and 30 U/mL recombinant human IL-2 (MCE, Cat# HY-P7037) for 24 h prior to co-culture. .. Levels of TNF- α and IFN- γ in supernatants from cocultures of PBMCs with Panc-1 cells were measured by ELISA (Cloud-Clone, Cat# USEA133Hu, Cat# USEA049Hu) to determine the cell ratio that best recapitulates an “immune-hot” cytokine milieu.

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Article Title: ERRα-ETV5 axis drives PD-L1 upregulation and immune escape in gallbladder cancer.
    Article Snippet: Gallbladder cancer (GBC) is a highly aggressive malignancy with a poor response to immune checkpoint blockade (ICB), highlighting an urgent need to understand the mechanisms of immune evasion.. We identified the orphan nuclear receptor ERRα as a critical regulator of the immunosuppressive tumor microenvironment in GBC.. Mechanistically, we discovered that ERRα transcriptionally upregulates the ETS-family transcription factor ETV5, which in turn directly binds to and activates the PD-L1 (CD274) promoter, establishing a novel ERRαETV5-PD-L1 signaling axis.

    Article Title: ALOX15 exerts tumor-suppressive role in head and neck squamous cell carcinoma via ERK pathway.
    Article Snippet: CD8+ T cells were subsequently isolated from the PBMCs using a human CD8+ T Cell Isolation Kit (immocell, IMPHK002) according to the manufacturer’s instructions. .. The purified CD8+ T cells were activated with human CD3/ CD28 T Cell Activator Magnetic Beads (immocell, IMCD15) and maintained in complete RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 10 ng/mL recombinant human IL-2 (MCE, HY-P7037). ..

    Article Title: Integrated Single-Cell Profiling Reveals TL1A as a Biomarker and Driver of Type 2 Inflammation via Macrophage-Dependent Immunoregulation in Asthma
    Article Snippet: The transfection was performed with plasmid/siRNA (GenePharm, Shanghai, China) using EndoFectinTM-Max (GeneCopoeia) according to the manufacturer’s instructions. .. The recombinant proteins, small molecular compound or inhibitor were purchased as indicated: recombinant mouse IL-13, IL-33, and TSLP protein (MCE); recombinant human IL-2, IL-4, TNF-α and IFN-γ protein (Abbkine); Poly(I:C) (Selleck); LPS D ow nloaded from https://spj.science.org on M arch 11, 2026 20 (Sigma-Aldrich); IL-1β (Thermo Fisher); small molecule inhibitors BAY11-7082, LY294002, PD98059, SB203580 and SP600125 (Selleck). ..

    Article Title: Integrated Single-Cell Profiling Reveals TL1A as a Biomarker and Driver of Type 2 Inflammation via Macrophage-Dependent Immunoregulation in Asthma
    Article Snippet: The transfection was performed with plasmid/small interfering RNA (GenePharm, Shanghai, China) using EndoFectin-Max (GeneCopoeia) according to the manufacturer’s instructions. .. The recombinant proteins, small-molecular compounds, or inhibitors were purchased as indicated: recombinant mouse IL-13, IL-33, and TSLP protein (MCE); recombinant human IL-2, IL-4, TNF-α, and IFN-γ protein (Abbkine); poly(I:C) (Selleck); lipopolysaccharide (Sigma-Aldrich); IL-1β (Thermo Fisher); and small-molecule inhibitors BAY11-7082, LY294002, PD98059, SB203580, and SP600125 (Selleck). ..

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer.
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Article Title: ALOX15 exerts tumor-suppressive role in head and neck squamous cell carcinoma via ERK pathway
    Article Snippet: CD8+ T cells were subsequently isolated from the PBMCs using a human CD8+ T Cell Isolation Kit (immocell, IMP-HK002) according to the manufacturer’s instructions. .. The purified CD8+ T cells were activated with human CD3/CD28 T Cell Activator Magnetic Beads (immocell, IMC-D15) and maintained in complete RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 10 ng/mL recombinant human IL-2 (MCE, HY-P7037). ..

    Inhibition:

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer.
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    In Vitro:

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer.
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Single Cell:

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer.
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Cell Culture:

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Article Title: Integrated multi-omics identifies a CD54 + iCAF-ITGAL + macrophage niche driving immunosuppression via CXCL8-PDL1 axis in cervical cancer.
    Article Snippet: .. To assess tumor inhibition in vitro, single-cell suspensions were prepared and cultured in RPMI-1640 medium supplemented with 100 IU/mL recombinant human IL-2 (MedChemExpress, Shanghai, China). ..

    Purification:

    Article Title: ALOX15 exerts tumor-suppressive role in head and neck squamous cell carcinoma via ERK pathway.
    Article Snippet: CD8+ T cells were subsequently isolated from the PBMCs using a human CD8+ T Cell Isolation Kit (immocell, IMPHK002) according to the manufacturer’s instructions. .. The purified CD8+ T cells were activated with human CD3/ CD28 T Cell Activator Magnetic Beads (immocell, IMCD15) and maintained in complete RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 10 ng/mL recombinant human IL-2 (MCE, HY-P7037). ..

    Article Title: ALOX15 exerts tumor-suppressive role in head and neck squamous cell carcinoma via ERK pathway
    Article Snippet: CD8+ T cells were subsequently isolated from the PBMCs using a human CD8+ T Cell Isolation Kit (immocell, IMP-HK002) according to the manufacturer’s instructions. .. The purified CD8+ T cells were activated with human CD3/CD28 T Cell Activator Magnetic Beads (immocell, IMC-D15) and maintained in complete RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 10 ng/mL recombinant human IL-2 (MCE, HY-P7037). ..

    Magnetic Beads:

    Article Title: ALOX15 exerts tumor-suppressive role in head and neck squamous cell carcinoma via ERK pathway.
    Article Snippet: CD8+ T cells were subsequently isolated from the PBMCs using a human CD8+ T Cell Isolation Kit (immocell, IMPHK002) according to the manufacturer’s instructions. .. The purified CD8+ T cells were activated with human CD3/ CD28 T Cell Activator Magnetic Beads (immocell, IMCD15) and maintained in complete RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 10 ng/mL recombinant human IL-2 (MCE, HY-P7037). ..

    Article Title: ALOX15 exerts tumor-suppressive role in head and neck squamous cell carcinoma via ERK pathway
    Article Snippet: CD8+ T cells were subsequently isolated from the PBMCs using a human CD8+ T Cell Isolation Kit (immocell, IMP-HK002) according to the manufacturer’s instructions. .. The purified CD8+ T cells were activated with human CD3/CD28 T Cell Activator Magnetic Beads (immocell, IMC-D15) and maintained in complete RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 10 ng/mL recombinant human IL-2 (MCE, HY-P7037). ..



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    Image Search Results


    Plasma level IL-2 in control (n=37) and PMOS (n=37) subjects and altered gene expression of JAKs in the total leukocyte RNA of 102 control and 102 PMOS subjects. A: Dot plot graphical representation of IL-2 level in the control and PMOS subjects. B-D: Dot and violin plot graphical representation of real-time gene expression level in JAK1, JAK2 and JAK3 . Each dot represents an individual subject. Each reaction was performed in triplicates and the ΔCt method was used to calculate the gene expression difference between control and PMOS subjects. 2 −ΔCt values of each subject are plotted on the Y-axis of the graph. Unpaired student’s T-tests were performed using GraphPad Prism 8 and p-value< 0.05 were set to be significant. Graph E and F: Dot and bar graph representation of relative gene expression level of JAK1, and JAK2 among the control and PMOS phenotype A, B, C, and D. The control group represented black dots, phenotype A in blue dots, phenotype B in orange dots, phenotype C in violet dots and phenotype D in yellow dots. One-way ANOVA was performed using GraphPad Prism 8 and p-value< 0.05 were set to be statistically significant.

    Journal: medRxiv

    Article Title: Targeting PTP1B and DUSP4 phosphatases to Boost Tregs: A Novel Therapy for Polyendocrine Metabolic Ovarian Syndrome (PMOS) Immune Dysfunction

    doi: 10.64898/2026.05.30.26354518

    Figure Lengend Snippet: Plasma level IL-2 in control (n=37) and PMOS (n=37) subjects and altered gene expression of JAKs in the total leukocyte RNA of 102 control and 102 PMOS subjects. A: Dot plot graphical representation of IL-2 level in the control and PMOS subjects. B-D: Dot and violin plot graphical representation of real-time gene expression level in JAK1, JAK2 and JAK3 . Each dot represents an individual subject. Each reaction was performed in triplicates and the ΔCt method was used to calculate the gene expression difference between control and PMOS subjects. 2 −ΔCt values of each subject are plotted on the Y-axis of the graph. Unpaired student’s T-tests were performed using GraphPad Prism 8 and p-value< 0.05 were set to be significant. Graph E and F: Dot and bar graph representation of relative gene expression level of JAK1, and JAK2 among the control and PMOS phenotype A, B, C, and D. The control group represented black dots, phenotype A in blue dots, phenotype B in orange dots, phenotype C in violet dots and phenotype D in yellow dots. One-way ANOVA was performed using GraphPad Prism 8 and p-value< 0.05 were set to be statistically significant.

    Article Snippet: After 24 hours of siRNA treatment, the cells were stimulated with 300IU of recombinant IL-2 (rIL-2) (#130-097-743, Miltenyi Biotech, Germany).

    Techniques: Clinical Proteomics, Control, Gene Expression

    FACS analysis showed an increase of CD4 +ve CD25 +ve CD127 low/- Treg population after PTP1B and DUSP4 (MKP-2) siRNA-based knockdown in PBMCs of PMOS (n=12) subjects. A: bar graph representation showing the gene expression of PTP1B and DUSP4 across the PTP1B, DUSP4, and both silenced PBMCs. B: Dot plot and histogram representation of gating strategy with P1 shows lymphocyte population, P2 shows CD4-FITC +ve population, P3 shows CD25-PE Cy7 +ve population and P4 shows CD127 low/- Treg population. Gating is based on histogram of unstained and stained population (FITC, PE Cy7 and Alexa Fluor 647 respectively). C: Dot plot representation of CD4-FITC +ve CD25-PECy7 +ve CD127-Alexa Fluor 647 low/- (P4) Treg population of all treatment conditions including; untreated, IL-2 treated, control siRNA silenced, control siRNA silenced with IL-2, PTP1B siRNA silenced without IL-2 and with IL-2, DUSP4 siRNA silenced without IL-2 and with IL-2, and PTP1B+DUSP4 siRNA silenced without IL-2 and with IL-2. D: Dot and truncated violin plot representation of the percentage of CD4 +ve CD25 +ve CD127 low/- Treg population in lymphocyte events across all the treatments. Each dot represents an individual subject. One-way ANOVA was performed using GraphPad Prism 8 and p-value< 0.05 were considered as statistically significant.

    Journal: medRxiv

    Article Title: Targeting PTP1B and DUSP4 phosphatases to Boost Tregs: A Novel Therapy for Polyendocrine Metabolic Ovarian Syndrome (PMOS) Immune Dysfunction

    doi: 10.64898/2026.05.30.26354518

    Figure Lengend Snippet: FACS analysis showed an increase of CD4 +ve CD25 +ve CD127 low/- Treg population after PTP1B and DUSP4 (MKP-2) siRNA-based knockdown in PBMCs of PMOS (n=12) subjects. A: bar graph representation showing the gene expression of PTP1B and DUSP4 across the PTP1B, DUSP4, and both silenced PBMCs. B: Dot plot and histogram representation of gating strategy with P1 shows lymphocyte population, P2 shows CD4-FITC +ve population, P3 shows CD25-PE Cy7 +ve population and P4 shows CD127 low/- Treg population. Gating is based on histogram of unstained and stained population (FITC, PE Cy7 and Alexa Fluor 647 respectively). C: Dot plot representation of CD4-FITC +ve CD25-PECy7 +ve CD127-Alexa Fluor 647 low/- (P4) Treg population of all treatment conditions including; untreated, IL-2 treated, control siRNA silenced, control siRNA silenced with IL-2, PTP1B siRNA silenced without IL-2 and with IL-2, DUSP4 siRNA silenced without IL-2 and with IL-2, and PTP1B+DUSP4 siRNA silenced without IL-2 and with IL-2. D: Dot and truncated violin plot representation of the percentage of CD4 +ve CD25 +ve CD127 low/- Treg population in lymphocyte events across all the treatments. Each dot represents an individual subject. One-way ANOVA was performed using GraphPad Prism 8 and p-value< 0.05 were considered as statistically significant.

    Article Snippet: After 24 hours of siRNA treatment, the cells were stimulated with 300IU of recombinant IL-2 (rIL-2) (#130-097-743, Miltenyi Biotech, Germany).

    Techniques: Knockdown, Gene Expression, Staining, Control